IBC > Journal Article
Journal Article Synopsis
IBC 2012, vol. 4, article no. 5, pp. 1-9 | doi: 10.4051/ibc.2012.4.2.0005
view 9011 | download 2169 | rating 7.5 | comment 0
Experimental method/Instrumentation (Bioimaging)
Eastern Staining: A Simple Recombinant Protein Detection Technology Using a Small Peptide Tag and Its Orthogonal Fluorescent Compound
Jae-Jung Lee1+, Jun-Young Kim2+, Duanting Zhai2, Seong-Wook Yun1 and Young-Tae Chang1,2,*
1
Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Biopolis, Singapore
2
Department of Chemistry, MedChem Program of Life Sciences Institute, National University of Singapore, Singapore
*Corresponding author
+These authors contributed equally to this work
received: June 01, 2012 ; accepted: June 12, 2012 ; published : June 15, 2012
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Synopsis

Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose “Estern staining”, as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming “immuno-staining” in many versatile applications.

Keywords : Western blot, Estern staining, fluorescence dye BDED, RC-tag, peptide chemical tags
Post-publication appraisal
Rate this manuscript
        Exceptional Highly recommended Recommended Fair Current rating: 7.5
Open discussion                       
(Open discussion is for 90 days after the initial publication)
:: Comments
Main text PDF(2903 KB)
(Print version)
Send to a friend
References
Reviewed by
- Seung Bum Park
- Young Eun Kwon
Edited by
- Hyun Joo
Author's Commentary
Export Citation
Bookmark
  StumbleUpon Facebook Connotea CiteULike twitter
PubMed
- Jae-Jung Lee
- Jun-Young Kim
- Young-Tae Chang
Google Scholar
- Jae-Jung Lee
- Jun-Young Kim
- Young-Tae Chang
Interdisciplinary Bio Central (IBC) ISSN : 2005-8543 | Contact IBC//
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution License.