IBC > Journal Article
Journal Article Synopsis
IBC 2010, vol. 2, article no. 1, pp. 1-9 | doi: 10.4051/ibc.2010.2.2.0001
view 11444 | download 3212 | rating 6.6 | comment 0
Review (Nano-bio and Nano-medical science and technology, Biomaterials/Biomimetics, Bioimaging)
Engineered Microdevices for Single Cell Immunological Assay
Jonghoon Choi1,*
1
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
*Corresponding author
received: March 22, 2010 ; accepted: April 27, 2010 ; published : April 29, 2010
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Synopsis

Microdevices have been used as effective experimental tools for the rapid and multiplexed analysis of individual cells in single-cell assays. Technological advances for miniaturizing such systems and the optimization of delicate controls in micron-sized space homing cells have motivated many researchers from diverse fields (e.g., cancer research, stem cell research, therapeutic agent development, etc.) to employ microtools in their scientific research. Microtools allow high-throughput, multiplexed analysis of single cells, and they are not limited by the lack of large samples. These characteristics may significantly benefit the study of immune cells, where the number of cells available for testing is usually limited. In this review, I present an overview of several microtools that are currently available for single-cell analyses in two popular formats: microarrays and microfluidic microdevices. Then, I discuss the potential to study human immunology on the single-cell level, and I highlight several recent examples of immunoassays performed with single-cell microdevice assays. Finally, I discuss the outlook for the development of optimized assay platforms to study human immune cells. The development and application of microdevices for studies on single immune cells presents novel opportunities for the qualitative and quantitative characterization of immune cells and may lead to a comprehensive understanding of fundamental aspects of human immunology.

Keywords : single cell assay, microfluidics, microwells, immune response
Post-publication appraisal
Rate this manuscript
        Exceptional Highly recommended Recommended Fair Current rating: 6.6
Open discussion                       
(Open discussion is for 90 days after the initial publication)
:: Comments
Main text PDF(3063 KB)
(Print version)
Send to a friend
References
Reviewed by
- Keun Woo Lee
- Hyun Joo
Edited by
- Sun Shim Choi
Author's Commentary
Export Citation
Bookmark
  StumbleUpon Facebook Connotea CiteULike twitter
PubMed
- Jonghoon Choi
Google Scholar
- Jonghoon Choi
Interdisciplinary Bio Central (IBC) ISSN : 2005-8543 | Contact IBC//
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution License.